This function can be used to load matrix market data in case genes were quantified by (i) counting all reads (for total RNA) and (ii) counting T-to-C mismatch reads (for new RNA)
Arguments
- genes
csv file (or URL) containing gene information
- cells
csv file (or URL) containing cell information
- new.matrix
Matrix market file of new counts
- total.matrix
Matrix market file of total counts
- detection.rate
the detection rate of T-to-C mismatch reads (see details)
- verbose
verbose output
Details
Metabolic labeling - nucleotide conversion RNA-seq data (such as generated by SLAM-seq,TimeLapse-seq or TUC-seq) must be carefully analyzed to remove bias due to incomplete labeling. We advice against counting read with and without T-to-C mismatches for quantification, and encourage using a statistical method such as GRAND-SLAM that properly deals with incomplete labeling.
To correct for some bias, a detection rate (as suggested by Cao et al., Nature Biotech 2020) should be provided. This detection rate defines, how much new RNA is detected on average using the T-to-C mismatch reads.