Compute absolute expression in a grandR object and puts the normalized data into a new slot
Usage
ComputeAbsolute(
data,
dilution = 40000,
volume = 10,
slot = "tpm",
name = "absolute"
)
Arguments
- data
the grandR object
- dilution
the dilution of the spikein transcript in the lysis reaction mix
- volume
the approximate volume of the lysis chamber (nanoliters)
- slot
the slot containing relative expression values
- name
the name of the new slot to put absolute expression values in
Value
a new grandR object with an additional slot
