Compute absolute expression using ERCC spike ins — ComputeAbsolute • grandR

Compute absolute expression in a grandR object and puts the normalized data into a new slot

Usage

ComputeAbsolute(
  data,
  dilution = 40000,
  volume = 10,
  slot = "tpm",
  name = "absolute"
)

Arguments

data: the grandR object
dilution: the dilution of the spikein transcript in the lysis reaction mix
volume: the approximate volume of the lysis chamber (nanoliters)
slot: the slot containing relative expression values
name: the name of the new slot to put absolute expression values in

Value

a new grandR object with an additional slot

See also